SIX1 antibody | knockout validation | Cell Signaling 12891

This is a knockout-validated antibody summary, based on the publication "A NIK-SIX Signalling Axis Controls Inflammation by Targeted Silencing of Non-Canonical NF-κB", as cited below [1] and "PAX-SIX-EYA-DACH Network modulates GATA-FOG function in fly hematopoiesis and human erythropoiesis" for western blot knockout validation (see figure 2f [2] ). Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Antibody information

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: SIX1

Catalog number: 12891

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues surrounding Pro249 of human SIX1 protein. Reacts with human, mouse, rat, and monkey. Suitable for western blot, immunoprecipitation, immunofluorescence/immunocytochemistry and flow cytometry.

Validation Method

Western blot

Sample

WT and SIX1/SIX2 knockout fibroblasts and in U-2 OS cells.

Figure

Characterization of fibroblasts and U-2 OS cells knock out of SIX1/SIX2. Please see Extended Data Figures 4d and 9c in the article [1].

References
  1. Liu Z, Mar K, Hanners N, Perelman S, Kanchwala M, Xing C, et al. A NIK-SIX signalling axis controls inflammation by targeted silencing of non-canonical NF-κB. Nature. 2019;: pubmed publisher
  2. Creed M, Baldeosingh R, Eberly C, Schlee C, Kim M, Cutler J, et al. PAX-SIX-EYA-DACH Network modulates GATA-FOG function in fly hematopoiesis and human erythropoiesis. Development. 2019;: pubmed publisher