This is a knockout-validated antibody summary, based on the publication "Metabolomics-assisted proteomics identifies succinylation and SIRT5 as important regulators of cardiac function", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: SIRT5

Catalog number: 8782

Summary: Rabbit monoclonal IgG against a recombinant protein specific to the full-length human SirT5 protein. Reacts with human, mouse and rat by western blot.

Western blot

Sirt5 WT and KO mouse hearts. Tissues were lysed in lysis buffer (50 mM Tris⋅HCl, pH 8.0, 150 mM NaCl, 1 mM EDTA, 1% Nonidet P-40, 5 mM nicotinamide, and protease inhibitor mixture).

5% BSA or milk in TBST (25 mM Tris⋅HCl, pH 7.4, 150 mM NaCl, and 0.1% Tween-20).

Diluted in 5% BSA or milk in TBST.

ECL Plus Western blotting detection reagents (GE Healthcare). Chemiluminescence was recorded by a Storm 860 Imager (Amersham Biosciences).

Please see Figure 6k in the article [1].

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.