This is a knockout-validated antibody summary, based on the publication "Acetylation accumulates PFKFB3 in cytoplasm to promote glycolysis and protects cells from cisplatin-induced apoptosis", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. HEK293T WT or SIRT1 knockout cells were treated with 25 μM glucose or 0 μM glucose for 5 h and immunoblotted with the indicated antibodies. From [1].

Rabbit monoclonal IgG

Company: Epitomics

Antibody: SIRT1

Catalog number: 1104

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to human SIRT1 aa 700-800 (C terminal). Reacts with human. Suitable for western blot, immunoprecipitation, immunocytochemistry/immunofluorescence, immunohistochemistry (paraffin and frozen) and flow cytometry.

Western blot

WT and HEK293T SIRT1-knockout cells. Cells were lysed in ice-cold NP-40 buffer containing 50 mM Tris-HCl (pH 7.4), 150 mM NaCl, 0.5% NP-40, 1 μg/ml Aprotinin, 1 μg/ml Leupeptin, 1 μg/ml Pepstatin, 1 mM Na3VO4, 50 mM NaF and 1 mM phenylmethylsulfonyl fluoride.

1:1,000 dilution.

Originally from Epitomics, it is now sold by Abcam (ab32441). Abcam purchased the company Epitomics.

The same clone (E104) is sold as Abcam ab32441; EMD Millipore 04-1091.