This is a knockout-validated antibody summary, based on two publications "MAVS activates TBK1 and IKKε through TRAFs in NEMO dependent and independent manner" (Figure 1, discussed below) [1] and "TBK-binding protein 1 regulates IL-15-induced autophagy and NKT cell survival" (Figure 2) [2]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot analysis of the indicated proteins in WT and TANK−/−NAP1−/−SINTBAD−/− triple KO 293T cells. From [1].

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: SINTBAD

Catalog number: 8605

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues surrounding Leu55 of human SINTBAD protein. Reacts with human, mouse and rat. It is predicted to react based on 100% sequence homology with monkey, and bovine. Suitable for western blot and immunoprecipitation.

Figure 2. Western blot analysis of SINTBAD (also called Tbkbp1) and other proteins in whole-cell lysates of IL-15-stimulated WT or Tbkbp1-KO thymic natural killer T cells. From [2].

Western blot

WT and TANK−/−NAP1−/−SINTBAD−/− triple KO 293T cells.