This is a knockout-validated antibody summary, based on the publication "Mitochondrial dysfunction remodels one-carbon metabolism in human cells", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Western blot of T-Rex293 cells lysates from wild-type and SHMT2 -/- and SHMT1/2 double knockout cells DKO). Probed with anti-SMHT2 antibody. From [1].

Rabbit polyclonal

Company: Thermo Fisher Scientific

Antibody: SHMT2

Catalog number: PA5-32228

Summary: Rabbit polyclonal antibody raised against a recombinant human SHMT2 fragment corresponding to a sequence within residues 1-483.

Reacts with human SHMT2.

Supplier recommended for Western blot, ICC, IF and IHC.

Western blot

Wild-type, SHMT2 knockout and SHMT1/2 double knockout T-Rex293 cells.

Anti-SHMT2.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.