This is a knockout-validated antibody summary, based on the publication "RANKL coordinates multiple osteoclastogenic pathways by regulating expression of ubiquitin ligase RNF146", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Mouse monoclonal IgG2a, Kappa

Company: Abnova

Antibody: SH3BP2

Catalog number: H00006452-M01

Summary: Mouse monoclonal antibody raised against a partial recombinant SH3BP2 (452 a.a. ~ 561 a.a). Reacts with human by western blot, immunofluorescence and ELISA.

Western blot

Osteoclasts derived from WT, Rnf146^fl/fl LysM-Cre, and Rnf146^fl/fl Sh3bp2^fl/fl LysM-Cre. Cells were lysed with RIPA buffer (50 mM Tris (pH 7.5), 150 mM NaCl, 1% NP40, 0.1% SDS, 0.25% sodium deoxycholate, 1 mM EDTA) supplemented with protease and phosphatase inhibitors.

5% BSA or 5% nonfat dried milk in PBST (PBS + 0.1% Tween-20).

Whole cell lysates from osteoclasts derived from WT, Rnf146^fl/fl LysM-Cre (KO), and Rnf146^fl/fl Sh3bp2^fl/fl LysM-Cre (dKO) were probed with the indicated antibodies for western blot analysis. Please see Figure 5d in the article [1].

The same clone (1E9) is sold as LifeSpan Biosciences LS-C198186; Abnova H00006452-M01; Sigma-Aldrich WH0006452M1.