This is a knockout-validated antibody summary, based on the publication "Tyrosine phosphatase SHP2 negatively regulates NLRP3 inflammasome activation via ANT1-dependent mitochondrial homeostasis", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Western blot of SHP2 in two groups of macrophages, i.e., peritoneal macrophages from conditional SHP2 knockout (cSHP2-KO) and wild-type (WT) mice. From [1].

Mouse monoclonal IgG1 (kappa light chain)

Company: Santa Cruz

Antibody: SH-PTP2

Catalog number: sc-7384

Summary: Mouse monoclonal IgG1 (kappa light chain) against an epitope mapping between amino acids 580-597 at the C-terminus of human SH-PTP2. Reacts with mouse, rat, human, avian and equine. Suitable for western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin), flow cytometry and ELISA.

Western blot

Wild-type and conditional SHP2 knockout mice peritoneal macrophages.

1:500 dilution overnight at 4 °C.

Horseradish peroxidase-coupled secondary antibody.

LumiGLO chemiluminescent substrate system.