This is a knockout-validated antibody summary, based on the publication "Small Glutamine-Rich Tetratricopeptide Repeat-Containing Protein Alpha (SGTA) Ablation Limits Offspring Viability and Growth in Mice", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Representative images of SGTA (red) protein detection in the prostate and testis of WT, Sgta+/− and Sgta−/− adult mice, from figure 4 [1].

Company: Santa Cruz

Antibody: SGTA (Small glutamine-rich tetratricopeptide repeat-containing protein α)

Catalog number: sc-373978

Summary: SGTA Antibody (G-11) is a mouse monoclonal IgG2b raised against amino acids 41-120 mapping near the N-terminus of SGTA of human origin, recommended for detection of SGTA of human origin by WB, IP, IF and ELISA

IHC, immunohistochemistry

Adult mouse tissue sections were incubated in 0.3% hydrogen peroxide (15 min).

Antigen retrieval: decloaker (10 mM citrate buffer (pH 6.5); 5 min at 125 °C and 10 sec at 90 °C).

successive 30 min blocking incubations in avidin/biotin block (Invitrogen, Mulgrave, Aus); Rodent Block M (Biocare Medical, Concord, US); and 10% goat serum.

overnight (4 °C).

red Alexa Fluor goat anti-mouse-594 (Invitrogen).

Sections were mounted (Prolong Gold anti-fade reagent with DAPI (Invitrogen) blue) and visualised by a Zeiss confocal microscope, ‘Zen’ software and Zeiss LDM700 with Lumen X-Cite Series 120PC camera.