This is a knockout-validated antibody summary, based on the publication "Small Glutamine-Rich Tetratricopeptide Repeat-Containing Protein Alpha (SGTA) Ablation Limits Offspring Viability and Growth in Mice", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

[enlarge]

Figure 1. From Figure 1 in [1]. Western blot of brain lysates from wild-type, SGTA +/-, and SGTA knockout mice probed with anti-SGTA antibody sc-373978. Actin (ACT) serves as a loading control.

Mouse monoclonal

IgG2a, kappa

Company: Santa Cruz Biotechnology

Antibody: SGTA

Catalog number: sc-373978

Summary: Mouse monoclonal antibody raised against residues 41-120 of human SGTA.

Supplier recommended for WB, IP, IF, IHC and elisa.

Reacts with human, mouse and rat SGTA.

Western blot

Immunohistochemistry

[enlarge]

Figure 2. From Figure 1 in [1]. Immunohistochemistry (prostate, ovary) with the SGTA antibody (sc-373978) in homozygous Sgta-null (−/−) tissue and heterozygous Sgta-null (+/−) tissue

Western blot: brain lysates from wild-type, SGTA +/-, and SGTA knockout mice.

Immunohistochemistry: formalin-fixed prostate and ovary from wild-type and SGTA knockout mice (benzamidine readout). Formalin fixed prostate and testis from wild-type, SGTA +/- and SGTA knockout mice (fluorescent readout).

Western blot: TBS containing 3% BSA and 0.1% Tween 20.

Immunohistochemistry: avidin/biotin and 5% goat serum (10% serum for fluorescent readout).

Western blot: anti-SGTA antibody diluted 1:500 in TBS containing 1%BSA and 0.1% Tween 20.

Immunohistochemistry: anti-SGTA antibody.

[enlarge]

Figure 3. From Figure 4 in [1]. Representative images of SGTA (red) protein in the prostate and testis of WT, Sgta+/− and Sgta−/− adult mice with sc-373978 antibody.

Western blot: HRP-conjugated anti-mouse IgG diluted 1:500 (Dako).

Immunohistochemistry: biotinylated anti-mouse IgG/streptavidin-conjugated HRP (diaminobenzamidine readout). AlexaFluor 594-conjugated goat anti-mouse IgG (fluorescent readout).

Western blot: Super Signal West Dura chemiluminescence reagent (ThermoFisher Scientific).

Immunohistochemistry: NanoZoomer Digital Pathology image scanner (diaminobenzidine readout) or Zeiss confocal microscope (fluorescent readout).

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.