This is a knockout-validated antibody summary, based on the publication "Small Glutamine-Rich Tetratricopeptide Repeat-Containing Protein Alpha (SGTA) Ablation Limits Offspring Viability and Growth in Mice", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. From Figure 1 in [1]. Western blot of brain lysates from wild-type, SGTA +/-, and SGTA knockout mice probed with anti-SGTA antibody. Actin (ACT) serves as a loading control.

Rabbit polyclonal

Company: Proteintech

Antibody: SGTA

Catalog number: 11019-2-AP

Summary: Rabbit polyclonal antibody raised against a GST fusion protein containing residues 1-313 of human SGTA.

Supplier recommended for WB, IP, IF, IHC and elisa.

Reacts with human, mouse and rat SGTA.

Western blot

Brain lysates from wild-type, SGTA +/-, and SGTA knockout mice.

TBS containing 3% BSA and 0.1% Tween 20.

Anti-SGTA diluted 1:1000 in TBS containing 1% BSA and 0.1% Tween 20.

HRP-conjugated anti-rabbit IgG diluted 1:1000 (Dako).

Super Signal West Dura chemiluminescence reagent (ThermoFisher Scientific).

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.