This is a knockout-validated antibody summary, based on the publications "Mitochondrial Permeability Uncouples Elevated Autophagy and Lifespan Extension", as cited below [1] and "Small tandem DNA duplications result from CST-guided Pol α-primase action at DNA break termini" for western blot knockout validation (figure 3d) [2]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: SGK3

Catalog number: 8156

Summary: Rabbit monoclonal IgG a synthetic peptide corresponding to residues surrounding Asp450 of human SGK3 protein. Reacts with human, mouse, rat and monkey. Suitable for western blot and immunoprecipitation.

Western blot

Control and Sgk1-3-TKO primary hepatocytes. Cell lysates were prepared in RIPA buffer (50 mM Tris-HCl pH 7.4,150 mM NaCl, 1% Triton x-100, 1% Sodium deoxycholate, 0.1% SDS,1 mM EDTA with proteinase inhibitor cocktail from Roche).

According to manufacturers’ protocols.

1:5,000 dilution goat-anti-rabbit-HRP conjugate (GE Healthcare) in 5% BSA dissolved in TBST.

West-Pico chemiluminescence substrate (Thermo Pierce).

Immunoblot of the indicated proteins in control and Sgk-TKO primary hepatocytes. Please see Suppl. Figure 7b in the article [1].