This is a knockout-validated antibody summary, based on the publication "Mitochondrial Permeability Uncouples Elevated Autophagy and Lifespan Extension", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rabbit monoclonal IgG
Company: Cell Signaling
Antibody: SGK2
Catalog number: 7499
Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues surrounding Lys331 of human SGK2 protein. Reacts with human, mouse and rat. Suitable for western blot.
Western blot
Control and Sgk1-3-TKO primary hepatocytes. Cell lysates were prepared in RIPA buffer (50 mM Tris-HCl pH 7.4,150 mM NaCl, 1% Triton x-100, 1% Sodium deoxycholate, 0.1% SDS,1 mM EDTA with proteinase inhibitor cocktail from Roche).
According to manufacturers’ protocols.
1:5,000 dilution goat-anti-rabbit-HRP conjugate (GE Healthcare) in 5% BSA dissolved in TBST.
West-Pico chemiluminescence substrate (Thermo Pierce).
Immunoblot of the indicated proteins in control and Sgk-TKO primary hepatocytes. Please see Suppl. Figure 7b in the article [1].
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