This is a knockout-validated antibody summary, based on the publication "SETDB1 modulates PRC2 activity at developmental genes independently of H3K9 trimethylation in mouse ES cells", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Rabbit polyclonal

Company: Santa Cruz Biotechnology

Antibody: SETDB1 / ESET

Catalog number: sc-66884

Summary: Rabbit polyclonal antibody raised against a polypeptide corresponding to residues 1-300 of human SETDB1 /ESET.

Supplier recommended for WB, IP, IF and elisa.

Reacts with human, mouse, rat, horse, cow and pig SETDB1 / ESET.

Note: this antibody will be discontinued December 15, 2016.

Western blot

Lysates of SETB1 conditional knockout mouse embryonic stem cells.

Super Block T20 (Thermo Scientific).

Anti-SETDB1 diluted 1:1000.

HRP-conjugated secondary antibody.

ECL reagent (Pierce).

See Supplemental Figure S5 from [1].

Awaiting reply.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.