This is a knockout-validated antibody summary, based on the publication "TITLE", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot of SET7/9 in WT and Set7/9−/− MEFs. Beta-actin was used as loading control. From [1].

Company: Cell Signaling Technology

Antibody: SET7/9

Catalog number: 2813

Summary: Rabbit polyclonal against a synthetic peptide corresponding to the human SET7/SET9 protein. Reacts with human, mouse, rat, and monkey. Suitable for western blot and immunofluorescence/immunocytochemistry.

Western blot

WT and Set7/9−/− MEFs.

1:1,000 dilution in 3% BSA diluted in PBS-T.

Horseradish peroxidase-conjugated secondary antibody (Jackson).

LumiFlash Ultima Chemiluminescent substrate (Visual Protein) and LAS-4000 mini (Fuji).

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.