This is a knockout-validated antibody summary, based on the publication "The critical role of SENP1-mediated GATA2 deSUMOylation in promoting endothelial activation in graft arteriosclerosis", as cited below [1] and the publication "CD34+KLF4+ Stromal Stem Cells Contribute to Endometrial Regeneration and Repair" for immunohistochemistry knockout validation (see figure 2h [2] ). Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Protein expression of SENP1 in MAECs from WT and SENP1-ecKO mice was determined by Western blotting. β-Actin was used as a loading control. From [1].

Rabbit monoclonal IgG

Company: Abcam

Antibody: SENP1

Catalog number: ab108981

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to human SENP1 aa 400-500. Reacts with human. Suitable for western blot, immunocytochemistry/immunofluorescence, immunohistochemistry (paraffin) and flow cytometry.

Western blot

Cells were lysed in 1 ml of cold lysis buffer containing 50 mM Tris-HCl, pH 7.6, 150 mM NaCl, 0.1% Triton X-100, 0.75% Brij96, 1 mM sodium orthovanadate, 1 mM sodium fluoride, 1 mM sodium pyrophosphate, 10 mg ml−1 aprotinin, 10 mg ml−1 leupeptin, 2 mM PMSF and 1 mM EDTA for 20 min on ice.

1:1,000 dilution overnight at 4 °C.

1:10,000 dilution horseradish peroxidase-linked anti-rabbit IgG (Jackson ImmunoResearch, #711-035-152) for 1 h at room temperature.

Chemiluminescence ECL detection kit (Biological industries).