This is a knockout-validated antibody summary, based on the publication "A CRISPR-Based Screen Identifies Genes Essential for West-Nile-Virus-Induced Cell Death", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Western blot of SEL1L knockout sublines of 293FT cells (clone S13 was chosen). Actin served as loading control. From [1].

Rabbit polyclonal IgG

Company: abcam

Antibody: SEL1L

Catalog number: ab78298

Summary: Rabbit polyclonal IgG against a synthetic peptide derived from within residues 300 - 400 of human SEL1L. Reacts with mouse and human and is predicted to work with rat and hamster. Suitable for western blot and immunohistochemistry (paraffin).

Western blot

CRISPR-mediated SEL1L knockout 293FT sublines. Cells were lysed with RIPA buffer supplemented with protein inhibitor cocktail (Roche Life Science).

5% skim milk in TBST overnight.

For 2 hours.

HRP-conjugated secondary antibody for 1 hour.

ECL western blotting substrate (Thermo Scientific), according to the manufacturer’s instructions.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.