This is a knockout-validated antibody summary, based on the publication "A CRISPR-Based Screen Identifies Genes Essential for West-Nile-Virus-Induced Cell Death", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Western blot of control and SEL1L-knockout in 293FT cells. Actin served as loading control. From [1].

Mouse monoclonal IgG1

Company: Santa Cruz

Antibody: SEL1L

Catalog number: sc-377350

Summary: Mouse monoclonal IgG1 against an epitope mapping amino acids 417-473 within an internal region of human Sel-1L. Recommended for detection of Sel-1L of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin) and ELISA. Also reactive with additional species, including equine, canine, bovine and porcine.

Figure 2. Western blot of control and SEL1L-knockout in Neuro-2a cells. Actin served as loading control. From [1].

Western blot

Control and CRISPR-mediated SEL1L knockout 293FT, Neuro-2a and HeLa cells. Cells were lysed with RIPA buffer supplemented with protein inhibitor cocktail (Roche Life Science).

Figure 3. Western blot of control and SEL1L-knockout in HeLa cells. Actin served as loading control. From [1].

5% skim milk in TBST overnight.

8 μg/ml for 2 hours.

HRP-conjugated secondary antibody for 1 hour.

ECL western blotting substrate (Thermo Scientific), according to the manufacturer’s instructions.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.