This is a knockout-validated antibody summary, based on the publication "Pyruvate carboxylation enables growth of SDH-deficient cells by supporting aspartate biosynthesis", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Goat polyclonal IgG

Company: Santa Cruz Biotechnology

Antibody: SDHB

Catalog number: sc-34150

Summary: Goat polyclonal IgG against an epitope mapping within an internal region of succinate dehydrogenase iron-sulfur protein of human origin. Recommended for detection of SDHB of mouse, rat, human and avian origin by western blot, immunoprecipitation, immunofluorescence and ELISA. Also reactive with additional species, including and equine, canine, bovine, and porcine.

Western blot

Sdhbwt/wt, Sdhbfl/fl and Sdhb knockout (Sdhbfl/fl,Cre) immortalized primary kidney epithelial cells. Cells were lysed in RIPA buffer supplemented with protein and phosphatase inhibitor cocktails.

10% non-fat milk in TBS-Tween.

1:500 dilution at 4°C overnight.

Anti-goat (926-32214, LI-COR) at 1:10,000 dilution.

Licor Odyssey scanner (channel, 700 and 800) and acquired using Image Studio 2.0.

Please see Supplementary Figure 1d in the article [1].

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.