This is a knockout-validated antibody summary, based on the publications "Succinate dehydrogenase B-deficient cancer cells are highly sensitive to bromodomain and extra-terminal inhibitors", as cited below [1], and "Succinate Mediates Tumorigenic Effects via Succinate Receptor 1: Potential for New Targeted Treatment Strategies in Succinate Dehydrogenase Deficient Paragangliomas" for western blot knockout validation (figure 2b) [2]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Control and SDHB knockout HCT116 cells were lysed 64 h after cell seeding, and expression levels of SDHB, SDHA, and β-actin were determined by western blotting. From [1].

Mouse monoclonal IgG2a, kappa

Company: Abcam

Antibody: SDHB

Catalog number: ab14714

Summary: Mouse monoclonal IgG2a, kappa against purified bovine mitochondrial complex II, with traces of complex III. Reacts with mouse, rat, hamster, cow, human, and zebrafish. Suitable for western blot, immunocytochemistry/immunofluorescence, immunohistochemistry (paraffin and frozen), and flow cytometry.

Western blot

Control and SDHB knockout HCT116 cells. Cells were washed with phosphate buffered saline (PBS) at 4°C and lysed with cell lysis buffer comprising 62.5 mM Tris-HCl (Wako), 10% glycerol (Wako), and 1% sodium dodecyl sulfate (SDS) (Wako).

Block Ace (DS Pharma Biomedical) in PBS containing 0.2% Tween 20 (PBS-T) or Starting Block T20 (PBS) Blocking Buffer (Thermo Scientific).

1:5,000 dilution overnight at 4°C.

Horseradish peroxidase (HRP)-conjugated secondary antibody (eBioscience) for 1 h at room temperature.

ImmunoStar Zeta (Wako) or ImmunoStar LD (Wako). Signals were visualized on an LAS-3000 image analyzer (Fujifilm) and quantified with Multi Gauge Ver. 3.1 (Fujifilm).

The same clone (21A11AE7) is sold as Abcam ab14714; Invitrogen 459230.