SDC4 antibody | knockout validation | Abnova PAB9045
DOI
//dx.doi.org/10.13070/ko.en.7.2149
Date
2017-02-17

This is a knockout-validated antibody summary, based on the publication "Syndecan-4 negatively regulates antiviral signalling by mediating RIG-I deubiquitination via CYLD", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

SDC4 antibody | knockout validation | Abnova PAB9045 figure 1
Figure 1. HEK293 Sdc4+/+ and Sdc4-/- cells were infected with SeV for 16 h, the cell membranes were isolated and analyzed by immunoblotting with the indicated antibodies.
Antibody information

Rabbit polyclonal

Company: Abnova

Antibody: SDC4

Catalog number: PAB9045

Summary: Rabbit polyclonal antibody raised against synthetic peptide corresponding to residues surrounding amino acids 184 of human SDC4. Reacts with human, mouse, rat and chicken. Suitable for western blot, immunohistochemistry (frozen and paraffin) and immunoprecipitation.

Validation Method

Western blot

SDC4 antibody | knockout validation | Abnova PAB9045 figure 2
Figure 2. Protein lysates isolated from Sdc4+/+ and Sdc4–/– mouse spleen were analyzed by immunoblotting with the indicated antibodies. From [1].
Sample

Sdc4+/+ and Sdc4-/- HEK293 cells and Sdc4+/+ and Sdc4–/– mouse spleen extracts.

Primary incubation

1:1,000 dilution.

References
  1. Lin W, Zhang J, Lin H, Li Z, Sun X, Xin D, et al. Syndecan-4 negatively regulates antiviral signalling by mediating RIG-I deubiquitination via CYLD. Nat Commun. 2016;7:11848 pubmed publisher