This is a knockout-validated antibody summary, based on the publication "The mitochondrial Nod-like receptor NLRX1 modifies apoptosis through SARM1", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Immunoblot of SARM1 in whole brain extracts taken from P0-4 pups of WT and Sarm1−/− (a) or cytosolic and mitochondrial fractions from WT or SARM1−/− CRISPR cells (b). Please see Figure S1 in the article [1].

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: SARM1

Catalog number: 13022

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues surrounding Pro324 of human SARM1 protein. Reacts with human by western blot, immunoprecipitation and immunofluorescence/immunocytochemistry.

Western blot

Brain extracts WT and Sarm1−/− mice; and WT and SARM1−/− HEK293T cells.

5% skim milk containing TBS/T (0.1% Tween 20).

Diluted in 5% skim milk containing TBS/T and incubated at 4 °C overnight.

Peroxidase conjugated anti-rabbit (Jackson Labs, 111-035-003) diluted in 5% skim milk containing TBS/T for 1 h at room temperature.