SAP 97 antibody | knockout validation | Santa Cruz sc-9961

This is a knockout-validated antibody summary, based on the publication "ARHGEF26 enhances Salmonella invasion and inflammation in cells and mice", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

SAP 97 antibody | knockout validation | Santa Cruz sc-9961 figure 1
Figure 1. DLG1 knockout was confirmed by both immunofluorescence (DLG1 in Gray/Red, Nucleus in Blue, Left) and immunoblotting whole cell lysates (Right). From [1].
Antibody information

Mouse monoclonal IgG1 κ

Company: Santa Cruz

Antibody: SAP 97

Catalog number: sc-9961

Summary: Mouse monoclonal against amino acids 1-229 of human SAP 97 protein (DLG1). Recommended for detection of SAP 97 of mouse, rat, human and canine origin by western blot, immunoprecipitation, immunofluorescence and immunohistochemistry (paraffin).

Validation Method

Western blot | Immunocytochemistry

Sample

Control and DLG1-KO MDCK cells.

Clone note

The same clone (2D11) is sold as Santa Cruz Biotechnology sc-9961; LifeSpan Biosciences LS-C35906; Invitrogen 35-9000.

References
  1. Bourgeois J, Wang L, Rabino A, Everitt J, Alvarez M, Awadia S, et al. ARHGEF26 enhances Salmonella invasion and inflammation in cells and mice. PLoS Pathog. 2021;17:e1009713 pubmed publisher