This is a knockout-validated antibody summary, based on the publication "The role of ryanodine receptor type 3 in a mouse model of Alzheimer disease", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Western blot analysis of brain lysates from WT and RyanR3-/- mice using C34 monoclonal antibody. Tubulin was used as a loading control. From [1].

Mouse monoclonal IgG1

Company: Thermo

Antibody: RyanR3

Catalog number: MA3-925

Summary: Mouse monoclonal IgG1 against partially purified chicken pectoral muscle ryanodine receptor. Reacts with amphibian, bovine, dog, chicken, fish, human, mustelid, mouse, primate, sheep, rabbit, and rat protein. Suitable for western blot, immunofluorescence, immunohistochemistry (frozen) and immunoprecipitation.

Western blot

Brain (hippocampal region) lysates from WT and RyanR3-/- mice. Tissue was homogenized on ice and proteins were solubilised at 4 °C for 1 h in 1% CHAPS lysis buffer on ice (1% CHAPS (Fisher Scientific (FS), BP57), 137 mM NaCl (FS, S641), 2.7 mM KCl (FS, P335), 4.3 mM Na2HPO4 (FS, S374), 1.4 mM KH2PO4 (FS, P380), pH 7.2, 5 mM EDTA (FS, BP2482), 5 mM EGTA (FS, O2783), and protease inhibitors (Roche, 04693116001).

1:500 dilution.

Horseradish peroxidase-conjugated anti-mouse secondary antibodies (Jackson ImmunoResearch, 115–035–146).

The same clone (34C) is sold as Novus Biologicals NB300-543; Abcam ab2868; Invitrogen MA3-925; Sigma-Aldrich R129; Developmental Studies Hybridoma Bank 34C.