Runx2 antibody | knockout validation | R&D Systems MAB2006
DOI
//dx.doi.org/10.13070/ko.en.7.2194
Date
2017-03-16

This is a knockout-validated antibody summary, based on the publication "Runx2 Deletion in Smooth muscle Cells Inhibits Vascular Osteochondrogenesis and Calcification but not Atherosclerotic Lesion Formation", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Antibody information

Rat monoclonal IgG2b

Company: R&D Systems

Antibody: Runx2

Catalog number: MAB2006

Summary: Rat monoclonal IgG2b against recombinant human RUNX2/CBFA1 isoform 2 Lys233-Tyr418. Reacts with human by immunocytochemistry.

Validation Method

Immunohistochemistry

Sample

Specimens were fixed with modified Clark’s fixative, processed, and embedded in paraffin for sectioning.

Secondary incubation

Biotinylated secondary antibodies, Vectastain ABC Elite kit (PK-6100, Vector Lab).

Detection

3, 3’-diaminobenzidine peroxidase substrate (D-0426, Sigma).

Figure

Please see Figure 1b,d,f in the article [1].

References
  1. Lin M, Chen T, Wallingford M, Nguyen N, Yamada S, Sawangmake C, et al. Runx2 Deletion in Smooth muscle Cells Inhibits Vascular Osteochondrogenesis and Calcification but not Atherosclerotic Lesion Formation. Cardiovasc Res. 2016;: pubmed