Rubicon antibody | knockout validation | Cell Signaling 8465

This is a knockout-validated antibody summary, based on the publication "Rubicon prevents autophagic degradation of GATA4 to promote Sertoli cell function", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Rubicon antibody | knockout validation | Cell Signaling 8465 figure 1
Figure 1. Immunoblotting of Rubicon in testes from mice of the indicated genotypes. n = 3. From [1].
Antibody information

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody:

Catalog number: 8465

Summary: Rabbit monoclonal antibody against a synthetic peptide corresponding to residues surrounding Leu210 of human Rubicon protein. Reacts with human and mouse. Suitable for western blot.

Validation Method

Western blot

Sample

WT and Rubicon-KO mouse testes.

Blocking agent

TBS-T containing 1% skim milk.

Primary incubation

1:1,000 dilution in TBS-T containing 1% skim milk.

Secondary incubation

1:2,000 dilution HRP-conjugated goat anti–rabbit IgG (Jackson ImmunoResearch, 111-035-003)

Detection

Luminata Forte (Merck Millipore) or ImmunoStar LD (Wako), and imaged using ChemiDoc Touch (Bio-Rad).

References
  1. Yamamuro T, Nakamura S, Yamano Y, Endo T, Yanagawa K, Tokumura A, et al. Rubicon prevents autophagic degradation of GATA4 to promote Sertoli cell function. PLoS Genet. 2021;17:e1009688 pubmed publisher