Rsf-1 antibody | knockout validation | EMD Millipore 05-727
DOI
//dx.doi.org/10.13070/ko.en.7.2248
Date
2017-04-08

This is a knockout-validated antibody summary, based on the publication "The chromatin remodeller RSF1 is essential for PLK1 deposition and function at mitotic kinetochores", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Rsf-1 antibody | knockout validation | EMD Millipore 05-727 figure 1
Figure 1. Western blot of RSF1 in cell lysates from WT and RSF1 knockout (KO) HeLa cells. From [1].
Antibody information

Mouse monoclonal IgG

Company: EMD Millipore

Antibody: Rsf-1

Catalog number: 05-727

Summary: Mouse monoclonal IgG against full length human Rsf-1. Reacts with human and mouse. Suitable for western blot, immunoprecipitation and immunocytochemistry.

Rsf-1 antibody | knockout validation | EMD Millipore 05-727 figure 2
Figure 2. Immunofluorescence images of human epithelial RPE1, control HeLa and RSF1 KO HeLa cells. RSF1 (green), ACA (red) and DAPI (blue). Scale bar, 5 μm. From [1].
Validation Method

Western blot | Immunocytochemistry

Sample

WT and RSF1 KO HeLa cells.

Blocking agent

ICC: 3% BSA for 1 h at room temperature

Primary incubation

WB: 1:1,000 dilution in TBST with 1% BSA.

ICC: 1:100 dilution in PBS with 3% BSA.

Secondary incubation

ICC: fluorescence-conjugated secondary antibody for 1 h.

Detection

ICC: Nikon A1R-A1 Confocal Microscope system with 60 × 1.4 numerical aperture (NA) Plan-Apochromat objective (Nikon Instrument Inc.) or LSM710 with 63 × 1.4 NA Plan-Apochromat objective (Carl Zeiss Zeiss) were used and analysed by the NIS elements C program or the ZEN 2011 program, respectively.

References
  1. Lee H, Park Y, Cho M, Chae S, Yoo Y, Kwon M, et al. The chromatin remodeller RSF1 is essential for PLK1 deposition and function at mitotic kinetochores. Nat Commun. 2015;6:7904 pubmed publisher