This is a knockout-validated antibody summary, based on the publication "USP26 functions as a negative regulator of cellular reprogramming by stabilising PRC1 complex components", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. RING1A knockout (KO) 293T cells were transfected with FLAG-USP26, cell extracts were harvested, immunoprecipitated with anti-FLAG antibody, and analyzed by western blotting using specific antibodies, as indicated. From [1].

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: Ring1A

Catalog number: 13069

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues surrounding Pro316 of human Ring1A protein. Reacts with human, and rat. It is predicted to react based on 100% sequence homology with guinea pig. Suitable for western blot, immunoprecipitation and immunofluorescence/immunocytochemistry.

Western blot

Control and RING1A knockout 293T cells. Cells were lysed in cell lysis buffer (50 mM Tris-HCl (pH 8.0), 150 mM NaCl, 1 mM EDTA, 1% Nonidet P-40, 2 mM MgCl2, 8 U benzonase, and 10% glycerol with protease inhibitor mixture) for 30 min.

1:1,000 dilution.

Luminata Crescendo Western HRP substrate (Millipore) was used for protein detection.