Rig-I antibody | knockout validation | Cell Signaling 4200

This is a knockout-validated antibody summary, based on the publication "DNA hypomethylation promotes transposable element expression and activation of immune signaling in renal cell cancer", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Antibody information

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: Rig-I

Catalog number: 4200

Summary: Rabbit monoclonal IgG a synthetic peptide corresponding to residues surrounding Gly719 of human Rig-I. Reacts with human and mouse. It is predicted to react based on 100% sequence homology with monkey, bovine, and pig. Suitable for western blot and immunoprecipitation.

Validation Method

Western blot

Sample

WT and RIGI-KO 786-0 cells.

Primary incubation

1:500 dilution.

Figure

Verification of MAVS-, MDA5-, and RIGI-KO cells in indicated 786-0 cell lines by immunoblot analysis. β-Actin was used as a loading control. Please see Figure 4a in the article [1].

References
  1. de Cubas A, Dunker W, Zaninovich A, Hongo R, Bhatia A, Panda A, et al. DNA hypomethylation promotes transposable element expression and activation of immune signaling in renal cell cancer. JCI Insight. 2020;5: pubmed publisher