Rig-I antibody | knockout validation | Cell Signaling 3743

This is a knockout-validated antibody summary, based on publications "The RNA genome of hepatitis E virus robustly triggers antiviral interferon response", as cited below [1], and "cGAS and DDX41-STING mediated intrinsic immunity spreads intercellularly to promote neuroinflammation in SOD1 ALS model" for western blot knockout validation (figure 3a) [2]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Antibody information

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: Rig-I

Catalog number: 3743

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues Lys652 of human Rig-I. Reacts with human, mouse, rat, and monkey. Suitable for western blot and immunoprecipitation.

Validation Method

Western blot

Sample

WT, RIG-I-/-, and RIG-I-/-/MDA5-/- MEF cells.

Secondary incubation

Anti-rabbit IRDye-conjugated antibodies (Stressgen, Victoria, BC, Canada).

Figure

The RIG-I protein level was detected in WT, RIG-I-/-, RIG-I and MDA5-/- MEF cells by western blot analysis. Please see Figure S8e in the article [1].

References
  1. Wang W, Wang Y, Qu C, Wang S, Zhou J, Cao W, et al. The RNA genome of hepatitis E virus robustly triggers an antiviral interferon response. Hepatology. 2018;67:2096-2112 pubmed publisher
  2. Tan H, Yong Y, Xue Y, Liu H, Furihata T, Shankar E, et al. cGAS and DDX41-STING mediated intrinsic immunity spreads intercellularly to promote neuroinflammation in SOD1 ALS model. iScience. 2022;25:104404 pubmed publisher