This is a knockout-validated antibody summary, based on the publications "RhoA, Rac1, and Cdc42 differentially regulate αSMA and collagen I expression in mesenchymal stem cells", as cited below [1], and "Macrophage-specific RhoA knockout delays Wallerian degeneration after peripheral nerve injury in mice" for immunohistochemistry and western blot (figure 1d,1e) [2]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Mouse monoclonal IgG1 (kappa light chain)
Company: Santa Cruz
Antibody: Rho A
Catalog number: sc-418
Summary: Mouse monoclonal IgG1 (kappa light chain) against amino acids 120-150 of human Rho A. Recommended for detection of Rho A of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin) and flow cytometry. Also reactive with additional species, including equine, bovine, porcine and canine.
Western blot
WT and RhoA-KO mesenchymal stem cells.
5% milk in TBS, 0.05% Tween (TBS-T) for 60 min.
Overnight at 4 °C.
Corresponding secondary antibodies (Jackson Immunoresearch) for 60 min.
Luminata Western horseradish peroxidase chemiluminescence substrate detection reagent (Millipore) for 1 min at room temperature. Luminescence was detected with medical X-ray film (Agfa).
Representative Western blotting of the indicated proteins in mesenchymal stem cells with knockout of RhoA. Please see Figure 3a in the article [1].
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