This is a knockout-validated antibody summary, based on the publication "One-step generation of conditional and reversible gene knockouts", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Mouse monoclonal IgG2a kappa light
Company: Santa Cruz
Antibody: Rfp2
Catalog number: sc-398129
Summary: Mouse monoclonal antibody specific for an epitope mapping between amino acids 34-51 near the N-terminus of human Rfp2 (TRIM13). Recommended for detection of mouse, rat and human. Suitable for western blot, immunoprecipitation, immunofluorescence and ELISA.
Western blot
Control and TRIM13-KO mouse embryonic stem cells (mESC). Cells were lysed in buffer containing complete protease-inhibitor cocktail tablets (11697498001, Roche).
1:500 dilution.
1:5,000 dilution horse anti-mouse IgG HRP conjugated (Cell Signaling).
ECL prime western blotting detection system (RPN2133, GE Healthcare).
Loss of TRIM13 protein expression following Cre recombination was confirmed by western blot (m). Please see Supplementary Figures 7m in the article [1].
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