This is a knockout-validated antibody summary, based on the publication "REV-ERBα influences the stability and nuclear localization of the glucocorticoid receptor", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Western blot of the indicated proteins in the cytoplasm and nuclear fraction of the liver of wild-type (WT) and Rev-Erbα−/− (Rev−/−) mice at zeitgebertime (ZT) 8 and ZT20. From [1].

Mouse monoclonal IgG1 (kappa light chain)

Company: Santa Cruz

Antibody: Rev-erbα

Catalog number: sc-100910

Summary: Mouse monoclonal IgG1 (kappa light chain) against recombinant Rev-erbα of human origin. Recommended for detection of Rev-erbα of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin) and ELISA.

Western blot

Livers from wildtype and Rev-Erbα−/− mice. Protein of liver tissue was extracted using RIPA buffer (50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 0.1% SDS, 1% Triton X-100, 0.5% sodium deoxycholate containing protease and phosphatase inhibitors).

0.5% dry milk in PBS-Tween 0.1%.

1:200 dilution overnight at 4°C.

Anti-mouse-IgG antibody conjugated to horseradish peroxidase (HRP).

Western Bright Quantum system (Advansta).

IHC was done using a different antibody: anti-Rev-Erbα antibody (ab174309, abcam, 1:100).