Rb antibody | knockout validation | BD Pharmingen 554136

This is a knockout-validated antibody summary, based on the publication "Cyclin D-CDK4 relieves cooperative repression of proliferation and cell cycle gene expression by DREAM and RB", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Rb antibody | knockout validation | BD Pharmingen 554136 figure 1
Figure 1. Knockout of RB from serum starved and contact arrested HFFs expressing sgRNA against control (sgCTL) and RB (sgRB1). Courtesy from the Authors [1].
Antibody information

Mouse monoclonal IgG1

Company: BD Pharmingen

Antibody: Rb

Catalog number: 554136

Summary: Mouse monoclonal IgG1 against human Rb (aa. 332-344). Reacts with human, mouse, rat, monkey, quail, mink (reported). Suitable for western blot, flow cytometry, bioimaging, immunoprecipitation, immunohistochemistry-formalin (antigen retrieval required), immunohistochemistry-frozen (reported).

Validation Method

Western blot

Sample

Control and RB-knockout human foreskin fibroblast cells (HFFs).

Blocking agent

5% milk in Tris Buffered Saline with 0.5% Tween-20.

Primary incubation

Overnight at 4°C.

Secondary incubation

Horseradish peroxidase-conjugated secondary antibodies (Rockland).

Detection

SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific) and imaged with G:BOX Chemi detection system (Syngene).

References
  1. SCHADE A, Oser M, Nicholson H, DeCaprio J. Cyclin D-CDK4 relieves cooperative repression of proliferation and cell cycle gene expression by DREAM and RB. Oncogene. 2019;38:4962-4976 pubmed publisher