Raptor antibody | knockout validation | Cell Signaling 2280

This is a knockout-validated antibody summary, based on the publication "Genetic inactivation of mTORC1 or mTORC2 in neurons reveals distinct functions in glutamatergic synaptic transmission", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Raptor antibody | knockout validation | Cell Signaling 2280 figure 1
Figure 1. A control (CTL) or Cre recombinase containing virus (CRE) was applied to hippocampal neurons from mice harboring floxed alleles at either the Raptor locus. From [1].
Antibody information

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: Raptor

Catalog number: 2280

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to the sequence of human Raptor. Reacts with human, mouse, rat, and monkey. Suitable for western blot, immunoprecipitation.

Validation Method

Western blot

Sample
Blocking agent
Secondary incubation

HRP-conjugated antibodies.

Detection

Enhanced chemiluminescent substrate (Pierce 32106).

References
  1. McCabe M, Cullen E, Barrows C, Shore A, Tooke K, Laprade K, et al. Genetic inactivation of mTORC1 or mTORC2 in neurons reveals distinct functions in glutamatergic synaptic transmission. elife. 2020;9: pubmed publisher