RalB antibody | knockout validation | Cell Signaling 90879

This is a knockout-validated antibody summary, based on the publication "The small G-protein RalA promotes progression and metastasis of triple-negative breast cancer", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

RalB antibody | knockout validation | Cell Signaling 90879 figure 1
Figure 1. Western blots demonstrating RALA and RALB expression in MDA-MB-231 CRISPR control (Ctrl), RALA CRISPR knockout (RALA-KO), and RALB CRISPR knockout (RALB-KO) cells.
Antibody information

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: RalB

Catalog number: 90879

Summary: Rabbit monoclonal antibody against a synthetic peptide corresponding to residues surrounding Glu142 of human RalB protein. Reacts with human. Suitable for western blot.

Validation Method

Western blot

Sample

Control and RALB-KO MDA-MB-231 cells.

Secondary incubation

Anti-rabbit IgG HRP (Cell Signaling Technologies, 7074).

References
  1. Thies K, Cole M, Schafer R, Spehar J, Richardson D, Steck S, et al. The small G-protein RalA promotes progression and metastasis of triple-negative breast cancer. Breast Cancer Res. 2021;23:65 pubmed publisher