Ral A antibody | knockout validation

Ral A antibody | knockout validation | BD Biosciences 610222

This is a knockout-validated antibody summary, based on the publication "RAL GTPases Drive Intestinal Stem Cell Function and Regeneration through Internalization of WNT Signalosomes", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Ral A antibody | knockout validation | BD Biosciences 610222 figure 1

Figure 1. H&E and Rala staining of regenerating crypts 3 days after irradiation. White arrows indicate regenerating crypts. Scale bar, 50 μm. From [1].

Antibody information

Mouse monoclonal IgG2a, κ

Company: BD Biosciences

Antibody: Ral A

Catalog number: 610222

Summary: Mouse monoclonal IgG2a, κ against human Ral A (aa. 35-206). Reacts with rat, dog, and chicken. Suitable for western blot, immunofluorescence, immunohistochemistry-formalin (antigen retrieval required). Not recommended for immunoprecipitation.

Validation Method

Immunohistochemistry

Sample

Control and conditional Rala-KO mouse intestine.

Primary incubation

1:500 dilution overnight at +4C.

References

Johansson J, Nászai M, Hodder M, Pickering K, Miller B, Ridgway R, et al. RAL GTPases Drive Intestinal Stem Cell Function and Regeneration through Internalization of WNT Signalosomes. Cell Stem Cell. 2019;24:592-607.e7 pubmed publisher

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