Rad51 antibody | knockout validation | Cell Signaling 8875

This is a knockout-validated antibody summary, based on the publication "PTEN and DNA-PK determine sensitivity and recovery in response to WEE1 inhibition in human breast cancer", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Rad51 antibody | knockout validation | Cell Signaling 8875 figure 1
Figure 1. Immunoblot analysis of MDA-MB-231 cell lines transduced with Cas9 and scrambled control gRNA or gRNAs targeting RAD51. GAPDH expression was used as loading control. From [1].
Antibody information

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: Rad51

Catalog number: 8875

Summary: Rabbit monoclonal IgG against recombinant protein specific to full-length human Rad51 protein. This antibody recognizes residues surrounding Leu140 of human Rad51. Reacts with human, mouse, rat, and monkey. Suitable for western blot and immunoprecipitation.

Validation Method

Western blot

Sample

Control and RAD51-KO MDA-MB-231 cell lines.

Blocking agent

5% nonfat milk or BSA in TBS-T

Detection

Western Lightning Plus-ECL Substrate Kit according to the manufacturer’s instructions (PerkinElmer).

References
  1. Brunner A, Suryo Rahmanto A, Johansson H, Franco M, Viiliäinen J, Gazi M, et al. PTEN and DNA-PK determine sensitivity and recovery in response to WEE1 inhibition in human breast cancer. elife. 2020;9: pubmed publisher