This is a knockout-validated antibody summary, based on the publication "Stress- And Ubiquitylation-Dependent Phase Separation of the Proteasome", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rabbit monoclonal IgG
Company: Cell Signaling
Antibody: Rad23B
Catalog number: 13525
Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues surrounding Ala140 of human Rad23B protein. Reacts with human and monkey. Suitable for western blot.
Western blot
Wild-type and RAD23B-KO HCT116 cells. Cells were lysed with buffer A with complete protease inhibitor cocktail (EDTA free) (Roche) for 30 min on ice.
5% non-fat milk for 30 min.
1 h at room temperature.
HRPconjugated goat anti-rabbit Ig (Jackson ImmunoResearch Laboratories) for 30 min at room temperature.
ECL Prime Western Blotting Detection Reagent (GE Healthcare) and analysed on an ImageQuant LAS4000 (GE Healthcare).
Wild-type, RAD23B-KO (RAD23BKO/KO) or UBE3A-KO (UBE3AKO/KO) HCT116 cells were stimulated with 0.2 M sucrose for 30 min and immunoblotted with the indicated antibodies. Please see Extended Data Figure 8a in the article [1].
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