This is a knockout-validated antibody summary, based on the publication "HUWE1 interacts with PCNA to alleviate replication stress", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Rabbit polyclonal

Company: Novus

Antibody: Rad18

Catalog number: NB100-61063

Summary: Rabbit polyclonal against an epitope mapping between residue 445 and 495 of human E3 ubiquitin-protein ligase RAD18. Reacts with human, squirrel and Rhesus macaque. Suitable for western blot, immunoprecipitation and PLA.

Western blot

Control and Rad18-knockout 293T cells.

Please see Figure 4g in the article [1].

HUWE1 (Novus NB100-652 and Bethyl A300-486A) were also KO validated (Fig 1). Authors have been contacted to check if both or only one of them.

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.