This is a knockout-validated antibody summary, based on the publication "Genetic ablation of Rac1 in cartilage results in chondrodysplasia", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Western blotting for Rac1 protein with Rac1 antibody BK035 was performed in cartilage, brain, kidney and lung extracts of Col2Cre-Rac1fl/fl mice (K) and Rac1fl/fl littermates without the Cre transgene (C). Courtesy of the authors.

Mouse monoclonal

Company: Cytoskeleton Inc

Antibody: Rac1

Catalog number: BK035 (kit) / ARC03 (individual antibody)

Summary: Mouse monoclonal that specifically recognised Rac1.

Supplier recommended for WB and ICC.

Reacts with human, mouse and rat Rac1.

Western Blot

RIPA extracts of mouse cartilage.

TBS containing 5% milk powder and 0.05% Tween 20.

Anti-Rac1 diluted 1:500 in blocking buffer.

ECL (Amersham Biosciences).

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.