This is a knockout-validated antibody summary, based on the publications "RhoA, Rac1, and Cdc42 differentially regulate αSMA and collagen I expression in mesenchymal stem cells", as cited below [1], and "Action potential-coupled Rho GTPase signaling drives presynaptic plasticity" for immunocytochemistry knockout validation (figure 4b) [2]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Mouse monoclonal IgG2b, κ
Company: BD Biosciences
Antibody: Rac1
Catalog number: 610650
Summary: Mouse monoclonal IgG2b, κ against human Rac1. Reacts with rat, human, mouse, dog, and chicken. Suitable for western blot, immunofluorescence, and immunohistochemistry. Not recommended for immunoprecipitation.
Western blot
WT and Rac1-KO mesenchymal stem cells.
5% milk in TBS, 0.05% Tween (TBS-T) for 60 min.
Overnight at 4 °C.
Corresponding secondary antibodies (Jackson Immunoresearch) for 60 min.
Luminata Western horseradish peroxidase chemiluminescence substrate detection reagent (Millipore) for 1 min at room temperature. Luminescence was detected with medical X-ray film (Agfa).
Representative Western blotting of the indicated proteins in mesenchymal stem cells with knockout of Rac1. Please see Figure 3a in the article [1].
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