This is a knockout-examinated antibody summary, based on the publication "Deletion of Rac1GTPase in the Myeloid Lineage Protects against Inflammation-Mediated Kidney Injury in Mice", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Figure 1. Immunoblotting of Rac1 in the bone marrow-derived macrophages (BMDM) from myeloid-selective Rac1 knockout (M-Rac1 KO) and flox control (M-Rac1 FC) mice. Actin was used as a loading control. The KO samples were from conditional knockout. The faint band in KO lanes could result from 1) incomplete knockout, or 2) contaminating cells, or 3) cross-reactivity of the antibody. From [1].

Antibody: Rac1

Catalog number: 05-389

Summary: Mouse monoclonal IgG2b antibody raised against full length human Rac. Used for immunohistochemistry, immunoprecipitation and western blotting for the detection of Rac1 protein of human, mouse and rat origin.

Company: Merck Millipore, now MilliporeSigma

Western blot

Bone marrow-derived macrophages (BMDM) from myeloid-selective Rac1 knockout (M-Rac1 KO) and flox control (M-Rac1 FC) mice. Whole cell lysates were extracted using MLB buffer containing 25 mM HEPES (pH7.5), 150 mM NaCl, 1% NP-40, 10% glycerol, 10 mM MgCl2, and Protease Inhibitor cocktail.

Dilution 1:2000

HRP-linked anti-mouse IgG (1:4000)

ECL, ECL Prime, or ECL Advance Western blotting detection system GE Healthcare

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.

The same clone (23A8) is sold as LifeSpan Biosciences LS-C85408; Abcam ab13048; EMD Millipore 05-389, 16-637, 16-319, FCMAB330F.