This is a knockout-validated antibody summary, based on the publication "B cell Rab7 mediates induction of activation-induced cytidine deaminase expression and class-switching in T-dependent and T-independent antibody responses", as cited below [1]. Another article "Control of RAB7 activity and localization through the retromer‐TBC1D5 complex enables RAB7‐dependent mitophagy" also indicates knockout validation for immunocytochemistry (figure 1b) and western blot (supplementary figure 1f) [2]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Rabbit monoclonal IgG

Company: Abcam

Antibody: Rab7

Catalog number: ab137029

Summary: Rabbit monoclonal IgG against a synthetic peptide corresponding to residues near the C terminal of human RAB7. Reacts with human, mouse, and rat. Suitable for western blot, immunocytochemistry/immunofluorescence, immunohistochemistry (paraffin) and flow cytometry. Unsuitable for immunoprecipitation.

Western blot | Immunocytochemistry

B cells from Rab7-KO (Igh+/Cγ1-creRab7fl/fl) and control (Igh+/Cγ1-creRab7+/fl ) mice. WB: Cells were lysed in buffer (20 mM Tris-Cl, pH 7.5, 150 mM NaCl, 0.5 mM EDTA, 1% (v/v) NP-40) supplemented with phosphatase inhibitors, including sodium pyrophosphate (1 mM), NaF (10 mM) and NaVO3 (1 mM), and a cocktail of protease inhibitors (Sigma-Aldrich). ICC: B cells were fixed with 2% paraformaldehyde.

ICC: 1% BSA.

ICC: at 25°C for 1 h.

ICC: Alexa Fluor647®-conjugated goat anti–rabbit Ab.

ICC: confocal microscope.

Please see Figure 2c,d in the article [1].