This is a knockout-validated antibody summary, based on the publication "Rab25 Deficiency Perturbs Epidermal Differentiation and Skin Barrier Function in Mice", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Representative immunohistochemistry images for Rab25 in 5-week old WT and Rab25 KO mice. From [1].

Mouse monoclonal IgG1

Company: Novus

Antibody: Rab25

Catalog number: NBP1-51599

Summary: Mouse monoclonal IgG1 against a purified recombinant fragment of Rab25. Reacts with human and mouse. Suitable for western blot, immunocytochemistry/immunofluorescence, immunohistochemistry, immunohistochemistry (paraffin), ELISA and flow cytometry.

Immunohistochemistry

WT and Rab25-KO mouse skin.

3% H2O2 for 30 min followed by protein block serum-free (X0909, DAKO) for 1-2 h at room temperature. M.O.M (BMK-2202, Vector Laboratories, Burlingame, CA, USA) reagent for 1 h was performed with mouse primary antibodies.

1:10,000 dilution overnight at 4°C.

HRP-conjugated secondary antibody (K4001/K4003, DAKO) for 15 min at room temperature.

The same clone (3F12) is sold as Novus Biologicals NBP1-51599; LifeSpan Biosciences LS-C169200; Abnova MAB10406; Invitrogen MA5-15587; ProMab 20430.