This is a knockout-validated antibody summary, based on the publication "Global Ablation of the Mouse Rab11a Gene Impairs Early Embryogenesis and Matrix Metalloproteinase Secretion", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

Mouse monoclonal IgG2a

Company: BD Transduction

Antibody: Rab11a

Catalog number: 610656

Summary: Mouse monoclonal IgG2a against human Rab11 aa. 86-207. Reacts with human, mouse, rat, chicken and dog. Suitable for western blot and immunohistochemistry. Not recommended for immunoprecipitation.

Immunocytochemistry

Wild-type and Rab11anull mouse blastocysts.

1:200 dilution in blocking buffer overnight at 4 °C.

Fluorescently conjugated secondary antibodies for 2 h.

Zeiss 510 laser-scanning confocal microscope.

Please see Figure 6 in the article [1].

If the antibody described in this summary is a polyclonal antibody, since polyclonal antibodies are of limited quantity, please inquire the supplier whether any current polyclonal antibody with the same catalog number is exactly the same as the one described in this summary. Sometimes, different bleeds or different animals are used, usually with a different lot number. In such cases, the result in this summary may not apply to the new antibody with the same catalog number.