RUNX2 antibody | knockout validation | Cell Signaling 12556

This is a knockout-validated antibody summary, based on the publication "Core Binding Factors are essential for ovulation, luteinization, and female fertility in mice", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

RUNX2 antibody | knockout validation | Cell Signaling 12556 figure 1
Figure 1. Immunohistochemical detection of RUNX1 and RUNX2 in ovaries of control and mutant mice collected at 11 h post-hCG (n = 3 animals/genotype). Immune-positive staining for RUNX1 or RUNX2 proteins (yellow/green) was localized to granulosa cells of periovulatory follicles in wild-type mice. Arrowheads and arrows point to granulosa cells and cumulus cells of preovulatory follicles, respectively. The sections were counterstained with propidium iodide (red) for nuclear staining. Scale bars, 250 μm for all images. From [1].
Antibody information

Rabbit monoclonal IgG

Company: Cell Signaling

Antibody: RUNX2

Catalog number: 12556

Summary: Rabbit monoclonal antibody against a synthetic peptide corresponding to residues surrounding Ala273 of human RUNX2 protein. Reacts with human, mouse and rat. Suitable for western blot, immunoprecipitation (IP), immunofluorescence (immunocytochemistry), flow cytometry and Chromatin IP.

Validation Method

Immunohistochemistry

Sample

WT and Cbfb/Runx2-DKO granulosa cell mouse tissues.

Primary incubation

1:200 dilution at 4 °C according to the manufacturer’s instructions.

Secondary incubation

Alexa Fluor secondary antibodies (Life Technologies).

Detection

Eclipse E800 Nikon microscope.

References
  1. Lee Thacker S, Jeon H, Choi Y, Taniuchi I, Takarada T, Yoneda Y, et al. Core Binding Factors are essential for ovulation, luteinization, and female fertility in mice. Sci Rep. 2020;10:9921 pubmed publisher