This is a knockout-validated antibody summary, based on the publication "A shared Runx1-bound Zbtb16 enhancer directs innate and innate-like lymphoid lineage development", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Rat monoclonal IgG2a, kappa
Company: eBioscience
Antibody: RUNX1-PE
Catalog number: 12-9816-80
Summary: Rat monoclonal IgG2a, kappa against RUNX1. Reacts with human and mouse. Suitable for flow cytometry.
Intracellular Flow Cytometry
Control floxed, heterozygous and Runx1 KO mouse thymic CD4SP and NKT cells. Cells were fixed and permeabilized using the eBioscience Foxp3 Staining Buffer Set.
1:100 dilution.
Samples were analyzed on an LSRII (BD Biosciences) or sorted on fluorescence-activated cell sorting (FACS) Aria II (BD Biosciences), with doublet exclusion and DAPI staining of dead cells in most experiments. Data was analyzed by FlowJo (Tree Star).
Intracellular flow cytometry for Runx1 expression in thymic CD4SP and NKT cells. See Figure 7a From [1].
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