This is a knockout-validated antibody summary, based on the publication "ROMO1 is a constituent of the human presequence translocase required for YME1L protease import", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Mitochondria from HEK293T WT and ROMO1−/− cells were lysed and analyzed by SDS-PAGE and Western blot. From [1].

Mouse monoclonal IgG1

Company: Origene

Antibody: ROMO1

Catalog number: TA505580

Summary: Mouse monoclonal IgG1 against full length human recombinant protein of human ROMO1. Reacts with human by western blot, immunofluorescence and immunohistochemistry.

Western blot

Mitochondria from WT and ROMO1-KO HEK293T cells.

Either an infrared secondary antibody or secondary antibody coupled to HRP.

Enhanced chemiluminescence detection kit (GE Healthcare) on x-ray films.

The same clone (OTI2C12) is sold as Novus Biologicals NBP2-45607; OriGene TA505580, CF505580; LifeSpan Biosciences LS-C337919; Abcam ab236409; Invitrogen MA5-26167.