This is a knockout-validated antibody summary, based on the publication "Ribonuclease inhibitor 1 regulates erythropoiesis by controlling GATA1 translation", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).
Mouse monoclonal IgG2b (kappa light chain)
Company: Santa Cruz
Antibody: RNH1
Catalog number: sc-365783
Summary: Mouse monoclonal IgG2b (kappa light chain) against amino acids 1-135 mapping at the N-terminus of human RNH1. Recommended for detection of RNH1 of mouse, rat and human origin by western blot, immunoprecipitation, immunofluorescence, immunohistochemistry (paraffin) and ELISA.
Western blot
WT and RNH1-KO K562 cell line. Cells were resuspended in lysis buffer (20 mM Tris, pH 7.4, 150 mM NaCl, 1% Nonidet-P40, 10 mM EDTA).
Total protein lysates of WT and RNH1-KO K562 cells were analyzed by Western blot with the indicated antibodies. Please see Figure 7a in the article [1].
- If you are aware of any publication with knockout studies validating a monoclonal or recombinant antibody, either purchased from a supplier or developed by the author(s), please notify us through feedback.