This is a knockout-validated antibody summary, based on the publication "Molecular mechanism of ER stress-induced pre-emptive quality control involving association of the translocon, Derlin-1, and HRD1", as cited below [1]. Labome curates formal publications to compile a list of antibodies with unambiguous specificity within Validated Antibody Database (VAD).

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Figure 1. Deletion of RMA1 was confirmed by immunoblotting. From [1].

Mouse monoclonal IgG1 (kappa light chain)

Company: Santa Cruz

Antibody: RNF5

Catalog number: sc-81716

Summary: Mouse monoclonal IgG1 (kappa light chain) against full-length recombinant human RNF5 (RMA1). Recommended for detection of RNF5 of mouse, rat and human origin by western blot, immunoprecipitation and immunohistochemistry (paraffin).

Western blot

WT and RMA1-KO HEK293 cells.

5% skim milk in TBS-T (50 mM Tris-HCl pH 8.0, 150 mM NaCl and 0.05% Tween-20).

ECL system.